机构地区: 中国科学院西北高原生物研究所
出 处: 《分析测试通报》 1990年第1期52-56,共5页
摘 要: 讨论了纳克量级(ppb)的18种氨基酸在石英毛细管住气相色谱的分离和定量测定。用此法对胶原蛋白和卵清蛋白水解氨基酸进行了定量。结果的精确度为0.8~7.3%。氨基酸的衍生化采用N-TFA-butyl系统,分二步完成,第一步在酸性介质中用亚丁醇酯化;第二步是用三氟醋酐酰化。氨基酸回收率在85%以上。蛋白质水解在6N恒沸点盐酸中充氮气,在145±2℃加热4小时完成。 Amino-acids from acid hydrolysis of collagen and egg white protein were esterfied in ac- idic solution with n-butyl alcohol and then treated with trfluoroacetic anhydryde. The 18 am- ino-acids in the acylation solution thus obtatained were determined by capillary gas chromato- graphy.