机构地区: 中国农业大学食品科学与营养工程学院
出 处: 《食品科学》 2007年第4期133-137,共5页
摘 要: 为深入研究苹果皮渣果胶的提取技术,本文比较了盐酸水解法和纤维素酶、半纤维素酶对苹果皮渣果胶的提取效果。采用高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)测定可溶性果胶的单糖组分,并通过粘度计测定特性粘度,推导粘均分子量。结果表明,酶法提取的果胶,比酸法提取的得率高2~3倍,且溶解性好.酸法、纤维素酶、半纤维素酶三种方法提取的可溶性果胶粘均分子量分别为292600、122400和165200Da。 In order to deeply investigate the technology of pectin extraction, pectin samples were extracted from apple pomace with hydrochloric acid and two enzymes (eellulasc and hemiccllulase). Solub'dized pectin was characterized for monosaccharide composition, intrinsic viscosity and viscosity average molecular weight by high performance anion exchange chromatography with pulsed amporometric detection (HPAEC-PAD) and viscosity detection. Enzymic extraction methods gives higher yields and better solubility of solubilized pectin. Average viscous molecular weight of solubilized pectin with hydrochloric acid, cellulase and hemicellulase treatment is 292600, 122400 and 165200Da, respectively.