机构地区: 中南大学湘雅二医院
出 处: 《中国医学工程》 2007年第1期25-27,30,共4页
摘 要: 目的通过对大鼠肾小球系膜细胞(MsC)转染反义转化生长因子β1基因(TGF-β1),观察该细胞纤维连接蛋白(FN)及1型纤溶酶原激活物抑制剂(PAI-1)表达的改变。方法构建反义TGF-β1基因真核表达载体,采用脂质体进行基因转染MsC,用ELISA法检测转染系膜细胞上清液中FN和PAI-1的蛋白质水平,并与无转染的MsC对照组和空载体组比较其表达的变化。结果构建了大鼠反义TGF-β1基因真核表达载体,并将其转染Msc,与对照组相比转染48小时后细胞分泌FN和PAI-1蛋白质水平明显下降(P<0.05)。结论反义TGF-β1基因真核表达载体可从分子水平阻断MsC的FN及PAI-1表达,在肾小球硬化及肾小球肾炎的研究治疗中有一定的应用价值。 [Objective] To study the Fibronectin (FN) and plasminogen activator inhibitor 1 (PAI-1) secretion on cultured rat mesangial cells (MsC) transfected with antlsense TGF-β1 vector. [Methods] The recombinant TGF-β1 antisense eukaryotic expression vector was generated. Lipofectin method was used to transfect the antisense TGF-β1 vector into MsC, and ELISA analysis was used for detecting FN and PAI-I protein level. [Results] Recombinant TGF-β1 antisense eukaryotic expression vector was constructed successfully. The protein of FN and PAI-1 was inhibited 48 hours after the transfection (P 〈0.05) in comparision with the control group. [Conclusion] Recombinant TGF-β1 antisense eukaryotic expression vector can inhibit the secretion of FN and PAI-1, which may be effective in gene therapy for sclerosing glomerulonephritis and proliferative glomerulonephritis.
关 键 词: 转化生长因子 反义 肾小球系膜细胞 纤维连接蛋白 型纤溶酶原激活物抑制剂
领 域: [生物学]