作　　者： ; ; ; ; ; ; ;

机构地区： 湖南农业大学

出　　处： 《实验动物与比较医学》 2007年第1期41-44,共4页

摘　　要： 目的建立人食管癌SCID异位移植模型并对其转移性淋巴来源细胞进行筛选。方法将食管癌细胞系Eca-109细胞悬液注射至SCID小鼠胃壁。三个月后或动物频死时处死解剖,观察肿瘤在小鼠体内的生长转移情况,对肉眼可见的转移病灶先建立皮下移植模型后建立转移性细胞亚系。结果胃壁移植15只SCID小鼠,获得9只胃壁移植瘤动物,其中一只出现纵隔淋巴结转移和肺转移。取淋巴结转移灶部分移植至SCID动物皮下,在动物皮下传代两个月(共4代)后,进行常规组织块培养,初步建立细胞亚系,命名为LnF1/Eca-109。结论肿瘤胃壁移植是建立食管癌免疫缺陷动物自发性移植模型和筛选转移性细胞亚系的可行方法。 Objective To report a new method for the establishing spontaneously metastatic animal model of human esophagus carcinoma and the metastatic-lymph node-deriving subline. Methods SCID mice were used in the experiments. Human esophagus carcinoma cell line Eca-109 were injected into the gastric mucosa layers in mice with a concentration of （1-2）×10^6 in 0.2 ml solution. All mice were sacrifed 3 months later or when the mice were found under the condition of general failure. Tumor metastases were investigated pathologically, part of the metastastic lymph node were inoculated subcutaneously for the establishment of metastatic subline. Results We established 9 gastric implantation animal models, one of which were found to have lung and lymph node metastases. Part of the metastatic lymph node was implanted subcutaneously in mice, and after its survival in vivo for 4 passages, a new subline was established using primary tissue culture. Conclusion Gastric wall implantation is a feasible method for establishing spontaneous metastases animal model and metastatic subline of human esophagus carcinoma.

关 键 词： 食管癌 动物模型 肿瘤转移 细胞系 细胞亚系

领　　域： [生物学]