作　　者： ; ; ; ; ; ;

机构地区： 中国农业大学生物学院农业生物技术国家重点实验室

出　　处： 《西北农业学报》 2007年第2期41-45,共5页

摘　　要： 根据genebank中公布的pmi基因序列设计引物,通过PCR扩增从大肠杆菌中分离出6-磷酸甘露糖异构酶基因。将该基因替换植物表达载体pCAMBIA1300中的潮霉素抗性基因hpt,构建了以pmi为选择标记基因的植物表达载体pPMI。对水稻进行了基因枪转化和农杆菌转化,研究了筛选培养基中甘露糖浓度对抗性愈伤组织频率的影响。部分抗性愈伤组织已获得再生植株,PCR检测初步证明外源基因已转入植物细胞。 According to pmi gene sequence in genebank, the 6-phosphomarmose isomerase gene of Escherichia coli was amplified by PCR. Based on pCAMBIA1300, the plant expression vector with pmi was constructed by substituting pmi for hygromycon resistance gene. Rice transformation was conducted by Microprojectile bombardment and by Agrobacteriurn mediated pathway. Research had been done on the percentage of resistant calli influenced by mannose concentration in selection medium. Some of the transgenic plants had been obtained . Transformation was confirmed by PCR.

关 键 词： 磷酸甘露糖异构酶基因 甘露糖 安全筛选标记 水稻 遗传转化

领　　域： [生物学]