作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 清华大学理学院化学系

出　　处： 《高等学校化学学报》 2007年第2期250-253,共4页

摘　　要： 采用HPLC-ESI-MSn分析得到了复方合煎液中有效部分提取物的指纹图谱数据,通过比对找出对复方指纹谱有贡献的有效成分峰.再用质谱差谱方法处理多级质谱数据,提取不同类型的中性丢失,实现了对复方有效成分化合物的快速分类鉴定.用该法研究了复方柴苓汤有效部分黄芩总黄酮和柴胡总皂苷. The constituents in effective groups of Chinese multiherb remedy possess the similar chemical and structural characters, and the same neutral loss under collisional induced dissociation （CID）. Based on this, the fingerprints of the multiherb remedy and the effective extracts were established by using HPLC-ESI-MS^n in this paper, the information of retention time, molecular weights and the fragment information was compared to confirm the attribution of effective constituents to the peaks in the fingerprint of Chinese multiherb remedy, then, the difference analysis of mass spectra was employed to deal with the fragment data for rapidly identifying the effective constituents according to the neutral loss. Taking Chinese multiherb remedy Chailin-tang for example, its effective fractions flavonoids in Radix Scutellariae and Saikosaponins in Radix bupleuriand were studied.

关 键 词： 质谱差谱方法 液相色谱 质谱 中药复方 有效部分 柴苓汤

领　　域： [理学] [理学]