作　　者： ; ;

机构地区： 浙江大学生物系统工程与食品科学学院食品科学与营养系

出　　处： 《农业生物技术学报》 2007年第1期138-141,共4页

摘　　要： 对70L发酵罐内Bacillus sp.EL31410分批发酵动力学模型进行了研究。在Logistic方程和Luedeking-Piret方程的基x础上,建立了弹性蛋白酶分批发酵动力学模型。根据实验数据确定了模型参数,得到菌体生长动力学模型为dx/dt=0.180(1-x/9.77)x,弹性蛋白酶生产动力学模型为dp/dt=-dx/dt=4.278x以及葡萄糖消耗动力学模型-ds/dt=0.03(dx/dt)-7.53x。将模型预测值与实验值进行比较,结果表明模型具有良好的适用性。 The kinetic models for elastase batch cultivation in a 70 L fermentor by Bacillus sp. EL31410 were discussed. Based on the Logistic and Luedeking-Piret equation, the mathematical dynamic models were proposed for cell growth, product formation and substrate consumption. The parameters of the kinetic models were established with experimental data. The models for cell growth,elastase formation and glucose consumption model were described by the following equations：dx/dt=0.180（1-x/9.77）x,dp/dt=-dx/dt＋4.278x and -ds/dt=0.03dx/dt-7.53x,respectively. It showed that the predict values of the models and experimental data were in a good agreement.

关 键 词： 弹性蛋白酶 分批培养 动力学模型 拟合

领　　域： [农业科学]