机构地区: 宁波大学理学院宁波市新型功能材料及其制备科学国家重点实验室培育基地
出 处: 《化学世界》 2006年第9期513-516,共4页
摘 要: 利用气相沉积法研制了基于TiO2凝胶固定化[Co(H2O)6](C4H4O4)·2C6H12N4·4H2O(CoL)修饰电极。研究了该修饰电极对谷胱苷肽(GSH)的催化活性,发现在pH7.5,0.1mol/LNaAc-HAc的缓冲溶液中,CoL对电氧化GSH浓度之间有较好的线性关系.线性范围为2×10^~6-1×10^-4mol/L,检测下限为8×10^-7mol/L,相对标准偏差为2.0%(cGSH=5×10^-6mol/L,n=6)。应用该法测定生物样品中谷胱苷肽的含量并与紫外分光光度法进行对照,结果一致。 [ Co(Ⅱ) ( H2O) 6 ] ( C4H4O4 )·2C6H12N4·4H2O(CoL) modified electrode was fabricated onto a titania sol-gel film through the vapor deposition method. It was applied to the electrochemical measurement of glutathione (reduced form) (GSH). A sensitive oxidation peak of GSH was observed on CoL modified electrode, in pH 7.5, 0.1 mol/L NaAc-HAc buffer solution. The oxidation peak current is linear with the GSH concentration between 2.0 × 10^-6 and 1 ×10^-4 mol/L. The detection limit is 8 × 10^-7 mol/L and RSD is 2.0% ( CGSH = 5 × 10^-4 mol/L, n = 6). The method was used for the analysis of GSH in biological samples and the results agreed well with those obtained with the established UV method.