作　　者： ; ; ; ;

机构地区： 广东海洋大学农学院

出　　处： 《生物技术通报》 2007年第1期113-116,132,共5页

摘　　要： 转基因植物的检测具有重要的意义。用抗草甘膦转基因大豆中的外源CaMV35S启动子、CP4 EPSPS和巢式PCR引物,应用PCR方法,从中扩增出预期大小的DNA片段。将扩增产物回收后测序,经同源性分析扩增产物为CaMV35S启动子和CP4 EPSPS的一部分序列。与常规PCR相比,巢式PCR在检测转基因大豆中具有更高的特异性。讨论了PCR检测过程中假阴性和假阳性的原因。 It is important to identify the transgenetic plant . The anticipated fragments were amplified from DNA of transgenetic plant resistant to Glyphosate by PCR method using the primers of CaMV35S promoters,CP4-EPSPS and nested PCR. The fragments were purified from the Agrose gel and sequenced The homology analysis proved that the fragments were parts of CaMV35S promoters and CP4-EPSPS. Nested PCR was more accurate in identification of transgenetic soybean than common PCR. The reasons of false negative and positive results were also discussed.

关 键 词： 抗草甘膦 转基因大豆 检测

领　　域： [农业科学] [农业科学] [农业科学]