作　　者： ; ; ; ; ; ;

机构地区： 华南农业大学生命科学学院

出　　处： 《食品工业科技》 2007年第1期107-109,128,共4页

摘　　要： 用海藻酸钙凝胶将黑曲霉产的酸性蛋白酶固定化,并研究了其若干性质。用4%的海藻酸钠与0.5%CaCl2制备的海藻酸钙凝胶将酶固定后,该固定化酶的活性回收率为33.33%。随浓度的增加,乙醇、丙三醇或SDS对这两个酶的抑制作用逐渐增强,而0～1.5mol/L盐酸胍、0～4mmol/L尿素却分别激活这两个酶。总之,固定化酸性蛋白酶对丙三醇浓度变化的敏感性较游离酶要大些,但对其它几种有机试剂浓度的变化的敏感性不及游离酶。 Acidic proteinase from Asperigillus niger, was immobilized in Ca-alginate gel produced by drop-wise addition of an aqueous mixture of sodium alginate and the enzyme to a hardening solution of a Ca^2＋ salt, and some of its properties were studied. The recovery ratio of the immobilized acidic proteinase activity was 33.33%, when 4% sodium alginate and 0.5% CaCl2 were used to produce Ca-alginate gel. In general, when the concentrations increased within the concentration range used in the article , the inhibitory effects of ethanol, glycerol and SDS on the activity of both the free enzyme and the immobilized one could become strong gradually, but 0-1.5mol/L guanidine hydrochloride and 0~4 mmol/L urea could activate the activity of both the enzymes respectively, in general, the immobilized acidic proteinase was more sensitive to the change of concentration of glycerol than the free one, but it was less sensitive to the changes of concentration of several kinds of organic reagent above than the free one.

关 键 词： 酸性蛋白酶 海藻酸钙 固定化 有机物 性质

领　　域： [轻工技术与工程] [轻工技术与工程]