机构地区: 暨南大学生命科学技术学院化学系
出 处: 《光谱学与光谱分析》 2007年第1期23-27,共5页
摘 要: 在4℃避光条件下,将藻蓝蛋白(简称PC)与AgNO3作用,通过UV,FS,FTIR等谱学方法研究了PC与Ag(Ⅰ)原位还原与纳米Ag(0)粒子形成的动态过程。结果显示:PC在615nm的特征吸收峰强度明显减弱,并随Ag(Ⅰ)浓度增加和时间延长单调降低;PC的荧光发射峰和荧光激发峰也均呈现衰减趋势。同步荧光光谱观察到纳米Ag(0)粒子形成的动态过程。用TEM观察到所形成的Ag(0)分散于PC表面,形成PC为核,纳米银为壳部分包覆的生物缀合物,粒子呈球形,有较窄的分布尺寸,粒径在15~30nm之间。 The interaction of Ag( Ⅰ) and Phycocyanin(PC for short) was investigated by ultraviolet-visible, fluorescence and infrared spectra under the condition of 4℃ and without light. The results showed that the characteristic absorption of PC at 615 nm decreased gradually with the increase in Ag( Ⅰ ) concentration and interaction time. Accordingly, its fluorescence emission peak and two fluorescence excitation peaks also decreased. Dynamic formation of nano-Ag(0) was also observed by synchronous fluorescence spectra, showing that Ag(0) was formed by the PC in situ reduction. It was observed by TEM that Ag(0) dispersed in PC, which formed a bioconjugate with PC as the core and nano-Ag(0) as the crust. The bioeonjugate was round and its diameter was about 15-30nm.
领 域: [生物学]