机构地区: 深圳出入境检验检疫局
出 处: 《植物保护》 2007年第1期117-120,共4页
摘 要: 本研究首次应用直接结合反转录实时荧光PCR技术(direct binding reverse transcription realtime PCR,DB-RT-Re-altime PCR)检测烟草环斑病毒,由于综合运用了PCR管吸附病毒外壳蛋白、病毒核酸分子杂交、高灵敏度实时荧光PCR技术的优点,从而使病毒检测在特异性、灵敏度、稳定性等技术指标上比传统的DAS-ELISA方法都有所提高,解决了烟草环斑病毒检测工作中由于隐症、干扰物质存在而影响检测结果的问题,为病毒检测提供了一个快速、简便有效的检测方法。 Direct binding reverse transcription realtime PCR technique was used for detecting Tobacco ringspot virus. For the advantages of combining capsid protein-absorption by PCR tubes and molecular hybridization of virus nucleic acids with high sensitivity of Real-Time PCR, this technique is better in detection specificity, sensitivity and stability than the traditional DAS-ELISA method. It also resolved the problems such as symptom-masking and interfering materials in detecting Tobacco ringspot virus.