作　　者： ; ; ; ;

机构地区： 西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室

出　　处： 《西北农林科技大学学报（自然科学版）》 2007年第1期24-28,共5页

摘　　要： 通过RT-RCR得到线虫f a t-1基因全长,将其N端亲水区克隆至原核表达载体pGEX-4T-2中,构建重组质粒pGEX-f a t1-N,将其转化大肠杆菌,并进行诱导表达。对表达产物G ST-f a t1-N融合蛋白进行纯化,制备其抗体,并对抗体效价进行了检测。结果表明,线虫f a t-1基因能在大肠杆菌中表达,制备的抗体能识别在原核表达系统内表达的G ST-f a t1-N融合蛋白,且效价很高,达到1∶107。 Caenorhabditis elegans fat 1 gene was obtained by RT-PCR,and the sequences of fat-1 gene N-terminal were cloned into fusion expression vector pGEX-4T-2 to generate recombinant plasmid pGEX- fat1-N. After expressing in Escherichia coli,the expressed product GST-fat1-N fusion protein was purified and used to immunize rabbit. The result showed C. elegans fat-1 gene could be expressed in E. coli,and the antibody against GST-fat1-N protein had a good affinity and selectivity by enzyme linked immunosorbent assay.

关 键 词： 基因 线虫 原核表达 抗体制备

领　　域： [生物学]