作　　者： ; ; ; ;

机构地区： 南京信息工程大学环境科学与工程学院

出　　处： 《山东农业大学学报（自然科学版）》 2006年第4期546-552,共7页

摘　　要： 我们已经建立了利用悬铃木叶片培养诱导植株的高频再生体系。本研究是在这一体系的基础上,以继代生长40天左右的组培苗顶部充分展开的叶片为受体,用含有反义磷脂酶Dγ(PLDγ)基因的农杆菌浸染叶片,通过在含有卡那霉素的培养基上进行抗性筛选,获得了抗性植株。对抗性植株进行了PCR和PCR-Southern杂交鉴定,证明反义PLDY基因已整合进悬铃木核基因组中。 We have previously established The high frequency regeneration system by using the young leaves of Platanus acerifoliaWiU as the culturing materia. Basing on this regeneration system, the ndedleaves - 8ultured about 40 day were used as the materials for gene transformation. The Agrobacteria tumerference containing pKY- LX7 plasmid harboring with antisense -PLD.y gene were used to infect the young leaves. Many kanamycin resistant planflets were obtained by culturing the infected leaves on the selection medium added with kanamycin. PCR and PCR -Southern tests showed that the antisense -PLDγ gene has been integrated into Platanus acerifoliaWilld genome.

关 键 词： 悬铃木 反义 基因 根癌农杆菌 转基因植株

领　　域： [交通运输工程]