作　　者： ; ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《华南理工大学学报（自然科学版）》 2006年第12期41-46,共6页

摘　　要： 在结构信息分析的基础上,选择具有特定作用位点的酶,进行酶解制备血管紧张素转化酶抑制肽(ACEIP)的研究,对以结构信息为基础的定向控制酶解制备模式进行了初步探讨.文中首先通过全略微分重叠计算,对所选择的11种抑制剂的结构信息进行分析,认为当肽链的N端为Asp、Glu,C端为Pro、Trp、Met、Phe、Lys时,对血管紧张素转化酶(ACE)的抑制效果较好;在此基础上,选择具有特定作用位点的酶,以罗非鱼肉为对象进行酶解制备ACEIP的研究,并对酶解产物进行分离和分析,发现其中含有Thr-Cys、Asp-Trp和Glu-Met,所得结果与理论分析结果相符,初步说明了以结构信息为基础的定向控制酶解制备模式的可行性. The aim of this research is to investigate the enzymatic preparation of angiotensin I-converting enzyme inhibitory peptide （ACEIP） on the basis of the structure information analysis and by choosing enzymes with preferential specificity, thus preliminarily giving a directional and controlled enzymolysis technology according to the structure information. In the investigation, the structure information of 11 selected angiotensin I-converting enzyme inhibitors was analyzed by means of the Complete Neglect of Differential Overlap （CNDO）. The results indicate that the oligopeptide possesses high inhibitory activity to angiotensin I-converting enzyme （ACE） when it is of residues Pro, Trp, Met, Phe and Lys in C-terminal position and when residues Asp and Glu in N-terminal position. Then, the enzymatic preparation of ACEIP from tilapia meat was conducted using enzymes with preferential specificity. The hydrolysate, in which Thr-Cys, Asp-Trp and Glu-Met were found, was finally extracted and analyzed. The results accord with the theoretical results, which preliminarily shows the feasibility of the directional and controlled enzymatic technology based on the structure information.

关 键 词： 血管紧张素转化酶抑制肽 酶解 结构信息 全略微分重叠计算

领　　域： [轻工技术与工程] [轻工技术与工程]