作　　者： ; ; ; ; ; ; ;

机构地区： 内蒙古农牧业科学院

出　　处： 《华北农学报》 2006年第6期11-15,共5页

摘　　要： 为进一步开展转基因研究创造条件,用限制性内切酶将葡萄糖氧化酶(GOD)基因从pMD/GO载体上切下,定向连接到植物表达载体pROKⅡ的CaMV35S启动子下游和NOS终止子上游,成功地构建了GOD基因植物表达载体pROK/GO。利用冻融法将此表达载体导入根癌农杆菌LBA4404中,提取转化质粒,经PCR和酶切鉴定表明,GOD基因植物表达双元载体构建成功。 This is the preparation for genetic transformation. Glucose oxidase gene was cut from pMD/GO with restraction enzymes. A plant expression vector pROK/GO was constructed. The fragment of Glucose oxidase gene in pROKⅡ was directively ligated downstream to CaMV35S promotor and upstream to NOS terminator. The plant expression vector was transformed into A. tumefaciens LBA4404 by the freeze-thaw method. The binary expression plasmid was further identified by PCR and restriction enzymes digestion. The results showed that the plant expression binary vector pROK./GO was constructed successfully.

关 键 词： 葡萄糖氧化酶基因 植物表达载体

领　　域： [生物学]