作　　者： ; ; ; ; ; ;

机构地区： 中国海洋大学水产学院海水养殖教育部重点实验室

出　　处： 《高技术通讯》 2006年第12期1306-1311,共6页

摘　　要： 设计了维生素D添加水平分别为0IU／kg（缺乏组），5×10^3IU／kg（正常组）和5×10^5IU／kg（过量组）的3种半精制饲料，在循环水养殖系统中用其饲养初始体重为（0．81±0．00）g，初始壳长为（16.42±0．03）mm的皱纹盘鲍240d，以期探讨维生素D对皱纹盘鲍生长和体组织抗氧化反应的影响。结果表明：与维生素D正常添加组相比，维生素D缺乏对皱纹盘鲍的增重率（WGR）和贝壳日增长（DISL）均没有显著差异（P〉0．05）；维生素D过量则显著降低皱纹盘鲍的增重率和贝壳日增长（P〈0．05）。皱纹盘鲍的不同组织（内脏团和肌肉）对维生素D缺乏或过量的敏感性不一致。维生素D缺乏显著降低内脏团中的GPX（谷胱甘肽过氧化物酶）和GST（谷胱甘肽-S转移酶）活力（P〈0．05），显著提高GR（谷胱甘肽还原酶）／GPX比值（P〈0．05），对CAT（过氧化氢酶）、SOD（超氧化物歧化酶）、GR活力以及CAT／SOD比值没有显著影响（P〉0．05）；而维生素D过量显著降低SOD和CAT活力的同时（P〈0．05），显著升高GPX和GR活力（P〈0．05）。肌肉中，维生素D缺乏对CAT、SOD、GST和CAT／SOD比值没有显著影响（P〉0．05），但显著降低GPX和GR活力（P〈0．05），同时显著升高GR／GPX比值（P〈0．05）；维生素D过量对CAT、GPX、GST、CAT／SOD和GR／GPX比值没有显著影响（P〉0．05），但显著升高SOD和GR活力（P〈0．05）。 A 240d growth experiment was conducted in a re-circulated water system to investigate the effects of dietary vitamin D on antioxidant responses of abalone Haliotis discus hannai Ino. Triplicate groups of juvenile abalone （initial weight： 0.81 ±0.00 g; shell length： 16.42 ±0.03 mm） were fed to satiation one of 3 semi-purified diets containing 0 IU/kg （deficiency）, 5 × 10^3 IU/kg （normal）, 5 × 10^3 IU vitamin D/kg diet （excess）, respectively. Results showed that compared with the normal group, vitamin D deficiency had no significant effect on the weight gain rate （WGR） and daily increment in shell length （DISL） （ P 〉 0.05） ; excessive vitamin D （5 × 10^3 IU/kg） significantly decreased the WGR and DISL of abalone （ P 〈 0.05）. The sensitivity of antioxidant enzymes in different tissues （viscera and muscle） of abalone was different to vitamin D deficiency or excess. Compared with the normal group, the activities of glutathione peroxidase （GPX） and glutathione-S-transferase （GST） were significantly decreased （ P 〈 0.05 ）, the ratio of GR（ glutathione reductase）/GPX was significantly increased （ P 〈 0.05）, while the activities of catalase （CAT）, superoxide dismutase （SOD）, GR and the ratio of CAT/SOD were not significantly affected in the viscera of abalone by vitamin D deficiency （ P 〉 0.05）. However, the activities of CAT and SOD were significantly decreased （ P 〈 0.05）, and GPX and GR was significantly increased （P 〈 0.05）, by vitamin D-excessive in the viscera of abalone （P 〉 0.05）. In muscle, vitamin D-deficiency had no significantly effects on the activities of CAT, SOD, GST and the ratio of CAT/SOD （ P 〉 0.05）, but significantly decreased the activities of GPX and GR and significantly elevated the ratio of GR/GPX （ P 〈 0.05） ; vitamin D-excessive had no significantly effects on the activities of CAT, GPX, GST and the ratio of CAT/SOD and GR/ GPX （ P 〉 0.05）, however, the activities of SOD

关 键 词： 皱纹盘鲍 维生素 抗氧化酶 贝类

领　　域： [农业科学] [农业科学]