作　　者： ; ; ; ; ; ;

机构地区： 华中师范大学生命科学学院

出　　处： 《华中师范大学学报（自然科学版）》 2006年第4期580-584,共5页

摘　　要： 采用RT-PCR方法,从稀有鮈鲫肌肉组织中分离和克隆出β-肌动蛋白基因cDNA部分序列,长度为1 029 bp,编码343个氨基酸残基.序列分析表明,该cDNA序列与其他物种β-肌动蛋白基因同源性非常高.RT-PCR能够检出该基因在稀有鮈鲫肌肉、眼、脑、心脏、肝、肠、鳃、睥、卵巢等组织中广普表达,在胚胎不同发育时期持续恒量表达.并基于已知的鱼类β-肌动蛋白基因序列构建了进化树. β-actin is a member of the actin family, which plays important roles in maintaining cytoskeleton, cell motility, cell division, intracellular movements and contractile processes. The partial sequence of β-actin cDNA of Gobiocypris rarus was cloned in present work from the muscles by reverse transcription-polymerase chain reaction （RTPCR）. This sequence consists of 1029 base pairs which encodes 343 amino acids. The amino acid identities of the corresponding partial sequences of the gene between the Gobiocypris rarus and other fishes were very high. Phylogenetic tree constructed based on β-actin nucleic acids showed the same relationships between Gobiocypris rarus and other fishes as that based on the mophology. RT-PCR analysis displayed consistent expression pattern of Gobiocypris rarus β-actin gene not only in different tissues, such as muscle, eye, brain, heart, liver, gut, gill, spleen and ovary, but also in various embryonic developmental stages, which suggests that β-actin gene is convinced to be used as a reference gene when other genes were studied in Gobiocypris rarus.

关 键 词： 肌动蛋白基因 稀有鮈鲫 克隆 组织表达 同源性比较

领　　域： [农业科学] [农业科学]