作　　者： ; ; ;

机构地区： 惠州学院生命科学系

出　　处： 《惠州学院学报》 2006年第6期51-55,共5页

摘　　要： 文章探讨了如何建立马铃薯茎尖分生组织试管苗的再生体系,包括外植体的获得、分生组织的切取和培养、茎尖分生组织诱导成苗以及快速繁殖等研究。实验共切取和培养110多个马铃薯茎尖。实验结果表明,(1)切取的茎尖当直径在0.3-0.6mm之间,并带有2-4个叶原基时成活率较高。(2)培养马铃薯茎尖分生组织的适宜培养基为MS+0.5mg/L IAA+0.1mg/L GA+0.04mg/L KT,茎尖易成活,也易分化出苗。(3)茎尖培养在温度为24-26℃,光照2000-2500 lx,每天光照12-15h的培养条件下,将生长得更好。 In this paper, how to establish the regeneration system in vitro from potato tip meristem is studied, including the planting method, tip mefistem isolation and culture, seedlings induction and rapid propagation. In the experiment, more than 110 potato Stem -apex were isolated and cultured. The results showed that, （1） When the size of the Stem- apex is between 0.3 mn, and 0.6 mm and carry 2- 4 leaf primordiums, the survival rate of Stem - apex is higher. （2） The suitable culture medium for stem - apex is MS ＋ 0.5mg/L IAA ＋ 0. 1mg/L GA ＋ 0.04mg/L KT, which enables Stem - apex to have comparative higher survival rate and differentiate well （3） Cultured in 24℃-26℃, 2000-2500 1x, and 12- 15 h per day, the Stem- apex will grow better.

关 键 词： 马铃著 组织培养 茎尖 再生体系

领　　域： [生物学]