作　　者： ; ; ;

机构地区： 新疆大学生命科学与技术学院

出　　处： 《生物技术》 2006年第6期60-62,共3页

摘　　要： 目的:为研究醉马草有毒成分的种类、特性及提取方法,达到最终纯化出有毒成分的目的。方法:结合系统预试验、全紫外波段扫描、多种方法对比提取效率、毒理实验等各种实验方法系统的对醉马草进行了研究。结果:醉马草含有生物碱、黄酮等多种成分,其中毒性成分主要是亲水部位的生物碱,中毒小白鼠表现为心跳呼吸加速、流泪、四肢无力嗜睡等症状,在醉马草整个生长期中,苗期所含总生物碱量最大,总生物碱在紫外波长为196nm、274nm和340nm有吸收峰,三种提取方法中乙醇煎煮法比较实用,正交实验法确定最佳提取工艺为80℃、50%的乙醇30倍体积下温育3h。 In order to separated the poisonous material of Achnathemm inebrians. Some experiments were done including systematic protested and contrasted the main components and quantifies of samples; Ultrasonic extraction, leaking method and ethanol decoction were compared; All the extracts were examined for toxicity to mice; Experiment of orthogonal design etc, As a result that the springtime sample has more the alkaloids than others; The proper measure absorbance were 196nm, 274nm and 340nm, The ethanol decoction method was the best, The toxiced mice were made heartbeat and breathing accelerating, toddle, lachrynmtion, sleepy- strongly, disability etc;The results showed that the optimized extracting condition were A3B2C3D3：on 80℃ boiling for 3 hours and using 25 times volume of 50% ethanol.

关 键 词： 醉马草 生物碱 正交设计 提取工艺

领　　域： [生物学]