作　　者： ; ; ; ;

机构地区： 华南农业大学理学院

出　　处： 《光子学报》 2006年第11期1709-1712,共4页

摘　　要： 通过与双光子荧光共焦成像过程比较,研究了非线性二次谐波的强度脉冲响应函数并对它进行频域分析,获得了横向、纵向截止频率和通频带.研究结果表明:二次谐波共焦成像具有更高的分辨率. The three-dimensional intensity pulse function of a confocal scanning microscope for secondharmonic wave is derived and compared with that of a two photon fluorescent confocal scanning microscope. By use of Fourier transformation, the three-dimensional intensity pulse function is theoretically investigated in frequency domain where the transverse and axial cut-off spatial frequencies, together with the transmission bands are given. The results show the optical resolution for second-harmonic wave is better than that for two-photon fluorescence. Such conclusion is also proved from the uncertainty principle.

关 键 词： 共焦显微镜 二次谐波 频域 分辨率

领　　域： [机械工程] [理学] [理学]