机构地区: 中国科学院西双版纳热带植物园
出 处: 《云南植物研究》 2006年第6期649-652,共4页
摘 要: 以小桐子(Jatrophacurcas)的胚芽、子叶、下胚轴、叶柄、叶片和茎段作为外植体,用不同浓度的6-苄基腺嘌呤(6-BA)和α-萘乙酸(NAA)对其进行愈伤组织的诱导和植株再生的研究。结果表明在MS培养基中加入5.0mg/L6-BA和1.0mg/LNAA对愈伤组织的诱导效果最好;加入5.0mg/L6-BA和0.1mg/LNAA对不定芽的诱导最为有效,加入0.1mg/L6-BA和1.0mg/LNAA有利于芽的生长;加入1.0mg/LNAA的1/2MS培养基对生根最为有利。 In this paper, plumules, cotyledons, hypocotyls, leaf blades, petioles and stalks of physic nut (Jatropha curcas L. ) were used as explants, and callus induction and plant regeneration were studied on MS medium contained different concentrations of 6-BA and NAA. The results showed that the MS medium with 5.0 mg/L BA and 1.0 mg/L NAA was the best for callus induction, and with 5.0 mg/L BA and 0.1 mg/L NAA, for formation of adventitious buds, and with 1.0 mg/L BA and 1.0 mg/L NAA, for bud growth, and that 1/2 MS medium with 1.0 mg/L NAA was the best for formation and growth of adventitious roots.
领 域: [生物学]