作　　者： ; ; ; ; ; ;

机构地区： 华南师范大学生命科学学院

出　　处： 《植物学通报》 2006年第6期703-707,共5页

摘　　要： 通过缩短DEAE-Cellulose柱长度,加快流速并采用pH8.8的80mmol.L-1Tris-HCl为洗脱液,可在9小时内快速地从菠菜、菜心和豆角绿叶中纯化得到乙醇酸氧化酶。该酶具高活性(54.6～197.0U.mg-1)及高等电点(pI>10.0)。产率为4.1%～71.5%,纯化倍数为21.6～122.68。经SDS-PAGE检测均有40kD带,表明3种植物乙醇酸氧化酶的亚基大小无区别。 By reducing the length of diethylaminoethanol-cellulose and increasing the pH in elution solution, we developed an improved method for purifying glycolate oxidase with high isoelectric value （pI 〉 10.0） from green leaves of Spinacia oleracea, Brassica parachinensis, and Phaseolus vulgaris. Purified glycolate oxidase showed high specific activity （54.6-197.0 U·mg^-1） and high pl （〉 10.0）. We achieved 21.6-122.68-fold purification and 4.1%-71.5% yield from crude solution during this purification procedure. SDS-PAGE of the glycolate oxidases revealed a 40-kD band, which indicates that the subunit molecular weight of glycolate oxidases in the 3 different plants was identical.

关 键 词： 乙醇酸氧化酶 纯化 菠菜 菜心 豆角

领　　域： [生物学]