作　　者： ; ; ; ; ;

机构地区： 江南大学食品学院食品科学与技术国家重点实验室

出　　处： 《中国生物工程杂志》 2006年第11期33-39,共7页

摘　　要： 利用鼠伤寒沙门氏菌针刺诱导家蝇幼虫表达抗菌肽,对抗菌肽的抑菌动力学进行研究,并通过抗菌肽对不同细菌动力学特性的研究出发对抗菌肽抑菌作用机制进行了探讨。研究发现抗菌肽样品的活性与作用时间有关,24h内出现一到两个活性峰,同一抗菌肽样品对不同细菌的抑菌动力学有差异,抗菌肽的抑菌动力学机制应该与它的抑菌作用机制有关。通过电镜观测、细胞磷代谢、紫外吸收物测定以及抗菌肽与细菌DNA相互作用结果可知,微生物诱导家蝇表达的抗菌肽样品不仅能够造成细菌细胞的快速坍塌破裂而且能够破坏细胞核心,与DNA结合。抗菌肽抑菌动力学的解释:微生物诱导产物中含有两部分抗菌肽,一部分抗菌肽能造成细胞膜的快速坍塌破裂形成第一个活性峰;另一部分抗菌肽可进入细胞,破坏细胞核心,造成紫外吸收物大量外泄形成第二个活性峰。 The antibacterial peptides from Musca domestica larvae was induced by injection with Salmonella typhimurium. From now on the investigation at antibiosis dynamics of antibacterial peptides had not been reported. The relation of antibacterial activity and action time of antibacterial peptides, which was called antibiosis dynamics, was investigated. The antibacterial peptide had different characters of antibiosis dynamics towards different bacteria. The characters was used to analyze the antibacterial mechanisms of the antibacterial peptides. There was one or two activity peaks in 24 hours of the antibacterial peptides sample. The same antibacterial peptides sample had different antibiosis dynamics property for different bacteria. The different antibiosis dynamics at E. coli ATCC25922 and E. coli JM109 which were both belonged to E. coli showed that the action time and mode of Musea domestica larvae antibacterial peptides were decided by the characteristic of bacteria cell. The results of scanning electron microscope （ SEM ） , cell phosphorus metabolism essay, cell ultraviolet absorbate detection and the interaction of antibacterial peptides and cell＇s DNA essay showed that the antibacterial peptides of Musca domestica larvae induced by microbe not only effected on the cellular membrane but also influenced the internal structure and destroyed with bacterial DNA. The interpretation of antibiosis dynamics is that one kind of antibacterial peptides made cellular membrane ruptured caused the first activity peak of antibiosis dynamics, the other kind of antibacterial peptides entered bacterial cell and reacted with genetic material caused the second activity peak.

关 键 词： 家蝇幼虫抗菌肽 抑菌动力学 抑菌机理

领　　域： [生物学]