作　　者： ; ; ; ;

机构地区： 河北农业大学

出　　处： 《中国农学通报》 2006年第11期54-56,共3页

摘　　要： 以引进爬山虎种子无菌苗为试验材料,对爬山虎组织培养中褐化现象进行了研究。试验结果表明,引进爬山虎种子无菌苗在组培诱导中褐化率高达89.3%,严重影响了脱分化和再分化。在培养基中添加不同浓度的AC、PVP、VC表明,VC是爬山虎组织培养中防止褐化较有效的方法,比AC和PVP的效果好,VC适宜的浓度为1.5g/L。 In this paper, the browning and antibrowning in the tissue culture of Parthenocissus tricuspidata was studied. The materials are aseptic stem segments with cotyledons from Canada. The results showed that aseptic stem segments with cotyledons appreared more browning, its browning rate reached even over 89.3%. Different concentrations of Vc or AC or PVP was added in medium, The results showed that Vc is the best method on antibrowning and its suitable concentration is 1.5 g/L.

关 键 词： 爬山虎 组织培养 褐化 褐化控制

领　　域： [生物学]