作　　者： ; ; ; ; ; ;

机构地区： 中国水产科学研究院黑龙江水产研究所

出　　处： 《水产学报》 2006年第5期701-706,共6页

摘　　要： A total of 85 interspecific hybrid F2 （Cyprinus carpio var.wuyuanensis ×Cyprinus pellegrini pellegrini） were cooled to specific temperatures and held at those temperatures over a maximum of 4 days in a water-recycled and temperature-controlled aquarium inside.As a result,the blood homeostasis of experimental fish changed violently as acute temperature changed from 16 ℃ to 10 ℃ and 4 ℃ at a rate of 1 ℃·h-1 according to the data we collected.Whole blood pH,also called extracellular pH（pHe） were very sensitive to temperature changes,where there was a significant difference between 10 ℃（7.41） and 16 ℃（7.17）（P<0.01）,compared to other values of hematology and serum chemistry.When the water temperature was continually decreased to an extreme temperature of 4 ℃,the content of Na+ of serum decreased remarkably in comparison with that of 10 ℃ and 16 ℃,which was 85.2 mmol·L-1,113.3 mmol·L-1 and 118.7 mmol·L-1,respectively.The values of hematology and serum chemistry also altered in gentle temperature changes of（10±2） ℃ and（4±2） ℃.Most values of serum chemistry and pH changed significantly,whereas the values of blood plasma changed slightly.pH was up slowly in 4 days at（10±2） ℃ and down slowly in 3 days at（4±2） ℃.A variety of values of serum chemistry changed remarkably both at（10±2） ℃ and（10±2） ℃,but the values of TP,TG and ALB only changed significantly at（4±2） ℃.These results distinguished at least two mechanisms involved in cold-induced stress in hybrid F2.Cold-induced pH changes resulted in other values altered.What’s more,pH correlated negatively with water temperature above 10 ℃,and the content of Na+.We also found that gentle temperature changes will be physiologically compensated for on day one at（10±2） ℃ and on day 2 at（4±2） ℃ in hybrid F2. A total of 85 interspecific hybrid F2 （ Cyprinus carpio var. wuyuanensis × Cyprinus pellegrini pellegrini ） were cooled to specific temperatures and held at those temperatures over a maximum of 4 days in a water-recycled and temperature-controlled aquarium inside. As a result, the blood homeostasis of experimental fish changed violently as acute temperature changed from 16 ℃ to 10 ℃ and 4℃ at a rate of 1 ℃ -h^-1 according to the data we collected. Whole blood pH, also called extracellular pH （pile） were very sensitive to temperature changes, where there was a significant difference between 10 ℃（7.41 ） and 16℃ （7.17 ） （ P 〈 0. 01 ）, compared to other values of hematology and serum chemistry. When the water temperature was continually decreased to an extreme temperature of 4 ℃, the content of Na^± of serum decreased remarkably in comparison with that of 10℃ and 16℃, which was 85. 2 mmol·L^-1, 113.3 mmol·L^-1 and 118.7 mmol·L^-1, respectively. The values of hematology and serum chemistry also altered in gentle temperature changes of （ 10 ± 2） ℃ and （4 ±2） ℃. Most values of serum chemistry and pH changed significantly, whereas the values of blood plasma changed slightly, pH was up slowly in 4 days at （ 10 ± 2） ℃and down slowly in 3 days at （4 ± 2）℃. A variety of values of serum chemistry changed remarkably both at （ 10 ± 2） ℃ and （ 10 ± 2）℃, but the values of TP, TG and ALB only changed significantly at （4 ± 2）℃. These results distinguished at least two mechanisms involved in cold-induced stress in hybrid F2. Cold-induced pH changes resulted in other values altered. What＇s more, pH correlated negatively with water temperature above 10 ℃, and the content of Na^± . We also found that gentle temperature changes will be physiologically compensated for on day one at （10±2） ℃and on day 2 at （4± 2） ℃ in hybrid F2.

关 键 词： 鲤 低温 胁迫 血液

领　　域： [农业科学]