作　　者： ; ; ; ; ; (叶卫）;

机构地区： 中山大学生命科学学院水生经济动物繁殖营养和病害控制国家重点学科专业实验室

出　　处： 《动物学报》 2006年第5期924-933,共10页

摘　　要： 尼罗罗非鱼(Oreochromis niloticus)雌雄鱼生长差异明显,为了探讨其原因,本文采用RT-PCR方法克隆了尼罗罗非鱼生长激素(Growthhormone,GH)及其受体(Growth hormone receptor,GHR)的cDNA序列,并应用半定量RT-PCR方法比较了雌、雄尼罗罗非鱼垂体GHmRNA、肝脏GHRmRNA、肌肉GHRmRNA的表达差异。序列分析表明:GH开放阅读框为615bp,共编码204个氨基酸;GHR开放阅读框为1908bp,共编码635个氨基酸。以RT-PCR方法研究了GH、GHR在各组织的分布情况,结果表明:GH仅在垂体中检测到有表达,而GHR在所检测的18种组织中均有表达,其中以肝脏、肌肉、性腺、下丘脑、胸腺表达量较高。以半定量RT-PCR方法进一步比较了雌、雄尼罗罗非鱼垂体GHmRNA、肝脏GHRmRNA、肌肉GHRmRNA的表达量,结果表明:雄鱼垂体GHmRNA和肝脏GHRmRNA的表达量均显著高于雌鱼,肌肉GHRmRNA的表达量则无显著差异,推测垂体GHmRNA和肝脏GHRmRNA表达的雌雄差异是尼罗罗非鱼雌雄生长差异的主要原因之一。 In order to study sex differences in growth in the the Nile tilapia Oreochromis niloticus, we cloned the cDANs encoding growth hormone and growth hormone receptor from pituitary or liver RNA by Reverse Transcription-Polymerase Chain Reaction （RT-PCR） and compared their expressions between male and female by Semi-Quantitative RT-PCR. The open reading fame of GH of Nile tilapia consists of 615 bp and encodes a putative protein of 204 amino acids, while the open reading fame of GHR consists of 1 908 bp and encodes a putative protein of 635 amino acids. By Semi-Quantitative RT-PCR, we measured GH and GHR expression in different tissues of Nile tilapia; GH expression was only detected in pituitary, while GHR expression occurred in all types of tissues we measured, and especially in liver, muscle, gonad, hypothalamus and thymus. Thus, by Semi-Quantitative RT-PCR, we further measured the mRNA levels of growth hormone in the pituitaries and growth hormone receptor in livers and muscles of male and female Nile tilapia growing at different rates. Significantly greater expression of GH in pituitaries and GHR in livers was measured in the males than in the females. These results show that different levels of GH and GHR transcription can explain the growth rate differences between male and female Nile tilapia .

关 键 词： 尼罗罗非鱼 生长激素 生长激素受体 雌雄表达差异

领　　域： [农业科学] [农业科学] [农业科学]