作　　者： ; ; ; ; ;

机构地区： 华南农业大学

出　　处： 《家禽科学》 2006年第11期11-13,共3页

摘　　要： 根据Genebank中I型鸭病毒性肝炎病毒的基因序列,设计合成了2对引物,以山东、江苏、广东、河南等地分离株的RNA为模板进行RT-PCR扩增,均得到预期大小的目的片段,两对引物的检测结果一致。并对其中一种方法的敏感性、特异性进行了检测,结果表明本试验建立的用于I型鸭病毒性肝炎病毒诊断的RT-PCR方法具有较高的敏感性和特异性,最低RNA模板检出量为100pg,只能特异的检测出I型DHV,不能检出NDV、IBDV、DPV和GPV。因此,所建立的RT-PCR检测技术具有特异、快速和敏感的特点,可用于鸭病毒性肝炎病毒的快速鉴别诊断。 A RT-PCR method for detecting the duck hepatitis virus I type was established with two pairs of primers designed and synthesized based on the gene of Genebank. The detection results of the samples were accordant by two pairs of primers.And the sensitivity,specificity tests were carried out.The re- suits showed that the sensitivity and specificity was very high. The RT-PCR method could detect 100pg RNA of the duck hepatitis virus isolated in Shandong,Jiangsu,Guangdong and Henan.But it couldn＂ t detect Newcastle disease virus,infectious bursal disease virus,duck plague virus and Goose paramyxoviridae.So the methode can be applied for the lastly diagnosis of Duck hepatitis virus Ⅰ type.

关 键 词： 鸭病毒性肝炎病毒 型 检测

领　　域： [农业科学] [农业科学] [农业科学]