作　　者： ; ; ; ; ; (陈世灿）; (方正刚）;

机构地区： 华南农业大学兽医学院

出　　处： 《中国兽医科学》 2006年第10期815-819,共5页

摘　　要： 根据分枝杆菌(mycobacteria)保守的插入序列IS1081设计4条特异性引物,建立了快速检测牛结核病的巢式PCR方法。该方法一次扩增的敏感性是1.35 pg,二次扩增的敏感性是1.35 fg。在对95份PPD阳性牛临床病料组织和23份血液样本的PCR检测中,用引物TB-Q1和TB-Q2做一次扩增,阳性检出率分别为36/95(37.5%)和5/23(21.7%);用引物TB-B1和TB-B2做二次扩增,阳性检出率分别为81/95(85.3%)和14/23(60.9%)。该方法作为辅助PPD试验的快速检测方法用于牛结核病的流行病学调查,具有重要的实用价值和应用前景。 Four specific primers, TB-Q1, TB-Q2,TB-B1 and TB-B2, were designed according to the published insert sequence IS1081 of mycobacteria and a nested PCR assay for the rapid detection of bovine tuberculosis was developed. Sensitivity of the first and second amplifications by the nested PCR was 1.35 pg and 1.35 fg, respectively. For 95 clinical samples and 23 blood samples from the positive cows by PPD test,the correspondence rates of the first PCR amplifications with primers TB-Q1 and TB-Q2 to the PPD test were 36/95（37.5 %） and 5/23（21.7 % ） respectively, and the correspondence rates of the second PCR amplifications with primers TB-B1 and TB-B2 to the PPD test were 81/95（85.3%） and 14/23（60.9%）, respectively. The results showed that the nested PCR assay is a useful technique for the rapid detection of bovine tuberculosis in supporting PPD test and epidemiological investigation.

关 键 词： 牛结核病 巢式

领　　域： [农业科学] [农业科学] [农业科学] [生物学]