作　　者： ; ; ; ;

机构地区： 广东省菌种保藏与应用重点实验室

出　　处： 《华南农业大学学报》 2006年第4期40-42,共3页

摘　　要： 从广东酸性土壤分离得到一种编号为2-1A1的丛枝菌根真菌.其孢子在土壤中单生,未见孢子果,椭圆形,黄色至褐色,孢子较大(170μm×230μm),孢壁一层,厚约11μm,M elzer s反应为黄色.其形态特征与Glomus flavispo-rum的特征描述相一致.提取其单孢子DNA,Nested PCR扩增其18S rDNA的NS31-AM1区,经序列测定,并在Gene-Bank上比对,进行系统进化树分析,结果表明,2-1A1与Glomussp.(DQ371674)的亲缘关系最近,序列同源性为98%.分子鉴定的方法只能将其鉴定到球囊霉属Glomus. Spores of an arbuscular mycorrhizal fungal which marked as 2-1A1 were isolated from the acid soil in Guangdong Province. The spores formed singly in soil, no sporocarp, ellipsoid, yellow to brown in color. Spore wall consisted by only one layer and 11 μm thick. Melzer＇s reaction was yellow. Strain 2-1A1 accorded with Glomusflavisporum in morphology. DNA was extracted from a single spore 2-1A1, then the NS31-AM1 region of 18S rDNA was amplified specifically by nested PCR. PCR products was sequenced and the DNA sequence was blasted on GeneBank. Phylogenetic tree revealed that strain 2-1A1 was closely related to Glomus sp. （DQ371674） ,with the similarity of 98%. Strain 2-1A1 was belong to the genus Glomus sp. by part of 18S rDNA sequence analysis.

关 键 词： 球囊霉属 形态 进化树 鉴定

领　　域： [生物学]