作　　者： ; ;

机构地区： 华南农业大学农学系

出　　处： 《华南农业大学学报》 1996年第4期27-34,共8页

摘　　要： 苦丁茶是近年来从野生资源中开发利用的非茶类植物，具适应性广、成材快、经济价值高等特点，可作代用茶，并有药用功能。用苦丁茶嫩梢茎切段及带柄叶片作外植体，在含２，４－Ｄ０５ｍｇ／Ｌ～４ｍｇ／Ｌ、ＫＴ１ｍｇ／Ｌ、蔗糖３０ｇ／Ｌ、琼脂７ｇ／Ｌ的改良ＭＳ培养基上，诱导产生愈伤组织，在同样培养基或添加不同附加物的培养基上继代培养。 Ilex kudingcha C. J. Tseng is a none tea plant in the nature, which has been tapping and utilizing from the natural resources in recent years. It has wide adaptability and grows rapidly. It has considerable economic importance, because it has some property of medicine, and it can be used as a substitute tea. Callus formation from explants of Iles kudingcha Tseng cutting shoots and leaves with stalk was induced on modified Murashige & Skoog basal medium supplemented with the 2,4 D 0.5 mg/L～4 mg/L,kinetin 1 mg/L, sugar 3 g/L and agar 0.7 g/L. Calli were subcultured on the same dedium or medium supplemented with other additions. Propogation and growth of calli were investigated.

关 键 词： 苦丁茶 组织培养 茎切段 改良 培养基

领　　域： [农业科学] [农业科学]