作　　者： ; ;

机构地区： 湖南大学环境科学与工程学院

出　　处： 《长沙理工大学学报（自然科学版）》 2006年第3期107-112,共6页

摘　　要： 在纤维素筛选培养基中加入了黄孢原毛平革菌稻草固态发酵的浸提液,并用这种培养基直接从自然环境中筛选了与黄孢原毛平革菌相容的菌株,在PDA和MEA平板上进行了进一步的相容性测试,选择出了生长稳定、可以与黄孢原毛平革菌较好共存的6株真菌.然后,对它们进行了固态发酵产酶的研究.对发酵体系中滤纸酶活(FPA)、木素过氧化物酶(L iP)、锰过氧化物酶(MnP)和漆酶(Lac)活力的测定结果表明,这种筛选出来的菌株组合可以部分增强酶的活性,完善体系中胞外酶的组成,其中编号为F05的菌效果最好,它在混合培养时,体系中的FPA和Lac都得到了加强,比单独培养F05和P.C.时的酶活分别提高了55.5%,24.1%和217%,146%. The compatible strains with P. Chrysosporium for biodegradation of straw were screened by modified cellulose agar which was added by extract obtained from solid-state fermentation with P. chrysosporium. 6 strains were selected by observing their morphologies on the PDA and MEA medium. Then these strains were inoculated into solid state medium made by straw with P. chrysos- porium, the pure cultures were set as blank. After the fermentation, samples were collected for measuring the FPA, LiP, Lac and MnP, the results shows that their enzyme were partially modi- fied. When F05 was cocultured with P. chrysosporium, compared to the pure cultures of F05 and P. chrysosporium, the FPA increased by 55.5%, 24.1%, respectively, and Lac increased by 217%, 146%, respectively. This simple and highly efficient method on screening combined strains was proved to be useful for studying how to improve the efficiency of straw biodegradation.

关 键 词： 白腐菌 共生菌株筛选 混合培养

领　　域： [环境科学与工程]