作　　者： ; ; ; ;

机构地区： 中国科学院广州能源研究所

出　　处： 《分析化学》 2006年第8期1197-1202,共6页

摘　　要： 连续流动式聚合酶链式反应（PCR）微流控装置是以低热容量的软薄膜加热器为基础构建3个恒温带，以聚四氟乙烯毛细管微通道为PCR反应体系组建而成。报道了在毛细管微通道中小牛血清蛋白（BSA）动力学表面钝化对PCR扩增的影响，讨论了PCR混合物在毛细管微通道中的流动速度对PCR扩增的影响，在15min甚至10min内成功扩增了长度为249bp的人类β-肌动蛋白片断，扩增时间仅10—15min，而传统PCR仪则需1～2h。 The polytetrafluoroethylene （PTFE） capillary-based continuous-flow polymerase chain reaction （PCR） microfluidics was constructed by using the flexible thin film heaters to form three constant temperature zones necessary for a PCR amplification, in which the PCR mixture could flow through the capillary microchannel continuously, thus leading to the successful completion of PCR exponential amplification. The effects of various concentrations of bovine serum albumin （BSA） in a 25 μL PCR mixture and flow rates of PCR mixture in the capillary microchannel, on continuous-flow PCR amplification, had been discussed in detail, where a PCR amplification of 249 base pair （bp） human β-actin gene fragments was successfully performed within 15 and even 10 minutes, which is several times faster than the reaction time required （1 -2 h） with the conventional PCR machine.

关 键 词： 毛细管 连续流动式聚合酶链式反应 微流控装置

领　　域： [理学] [理学]