作　　者： ; ; ; ; ;

机构地区： 中国海洋大学水产学院

出　　处： 《海洋学报》 2006年第4期115-120,共6页

摘　　要： 雄性牙鲆单独注射促黄体激素释放激素类似物(LHRH-A)(每千克质量的注射量,下同)25μg/kg或人绒毛膜促性腺激素(hCG)1 000 IU/kg,血浆睾酮(T)含量在注射后6 h大幅度降低,12h时达到最低值,以后逐渐回升;LHRH-A与hCG联合注射[LHRH-A(12.5μg/kg)+hCG(500 IU/kg)],注射后6 h血浆T含量剧烈下降,24 h时达到最低点,以后缓慢回升;对照组雄鱼血浆T含量随着采样进行而呈现降低趋势.雌鱼单独LHRH-A后,血浆雌二醇(E2)水平逐渐下降,24 h时达到最低值,30 h后略有回升;单独注射hCG后,血浆E2含量缓慢下降,30 h达到最低值;LHRH-A与hCG联合注射,注射后6 h血浆E2含量逐渐下降,24 h时达到最低点,30 h时缓慢回升.对照组雌鱼血浆E2水平也随着采样的进行而逐渐降低.结果表明:单独或联合注射LHRH-A和hCG降低了雌、雄牙鲆血浆E2和T含量,与对照组结果相同. When male Japanese flounders were injected with alone luteinizing hormone--releasing hormone analogue（LHRH-A） （25 g/kg） or human chorionic goandotropin（hCG） （1 000 IU/kg）, plasma testosterone（T） levels decreased significantly postinjection 6 hour, and occurred the lowest levels postinjection 12 hour, and somewhat increased postinjection 24 to 30 hour. Plasma T content declined abruptly postinjection 6 hour and appeared the least value of plasma T postinjection 24 hour in that male fish injected with LHRH--A plus hCG （12.5 g/kg＋500IU/kg）. At the same time, the plasma T levels of male fish in con- trol group also decreased as these fish treated with LHRH--A or hCG. The plasma estradiol（E2 ） levels decreased significantly postinjection 6 hour, and occurred the lowest levels postinjection 24 hour, and somewhat increased postinjection 30 hour, when female fish were injected with alone LHRH-A, whereas, the least plasma E2 levels occurred postinjection 30 hour in that female fish injected with alone hCG. Plasma E2 content decreased gradually postinjection 6 hour and appeared the least value of plasma E2 postinjection 24 hour in that female fish injected with LHRH--A plus hCG. On the mean while, the plasma estradiol levels of the fish in control group also declined as these fish treated with LHRH--A or hCG. These results suggestion that the injection with alone LHRH--A or hCG or combined LHRH--A and hCG failed to increase significantly plasma testosteroneand estradiollevels, on the contrary, suppressed testosteroneand estradiolconcentration in male and female Japanese flounder.

关 键 词： 牙鲆 性激素 生殖

领　　域： [生物学]