作　　者： ; ;

机构地区： 湘潭大学

出　　处： 《离子交换与吸附》 1996年第5期397-402,共6页

摘　　要： 用聚苯乙烯阴离子交换树脂作载体，采用吸附法将ａ一淀粉酶固定化，研究了固定化条件和固定化酶的性能。固定化条件为：酶浓度１０ｍｇ／ｍｌ，固定化ｐＨ６．８，吸附时间１６ｈ，吸附温度６℃。固定化酶的ｐＨ稳定性、热稳定性、贮存稳定性都有一定的提高。最适作用温度及ＰＨ较自由酶为高，Ｋｍ值为自由酶的１．６倍。同时也进行了固定化酶的操作稳定性研究。 ?Amylase was immobilized on polystyrene anion exchange resin as supportby means of adsorption. The conditions of immobilization of α- Amylase and theproperties of the immobilized enzyme were reported. The optimization for immobilizatlon was: enzyme concentration 10 mg/ml gtemperature 6 ℃, time 16h and PH6. 8 , respectively. The stabilities of the immobilized enzyme to temperature, PH,storag and its optimum term3erature and optimum PH were separately better thanthe free enzyme. The k. of the former was 1. 6 times that of the latter. The operaartioll stability of the former was also discussed.

关 键 词： 固定化 淀粉酶 聚苯乙烯 阴离子交换树脂

领　　域： [轻工技术与工程] [化学工程]