作　　者： ; ; ; ;

机构地区： 四川农业大学动物科技学院

出　　处： 《微生物学报》 2006年第4期649-652,共4页

摘　　要： 从发生急性流行性传染病的斑点叉尾肝、肾分离到一高致病性的菌株(CCF00024),经人工感染实验证实其为该病的病原菌。对该菌的形态、生理生化及16S rDNA序列分析结果表明,其为非发酵型,严格需氧,革兰氏阴性杆菌,极生多鞭毛,对除麦芽糖和甘露糖以外的多种糖类不能利用产酸,氧化酶阴性,DNA酶、蛋白酶、脲酶、赖氨酸脱羧酶阳性,MR阴性。在以该菌16S rDNA序列(GenBank登录号AY970826)和GenBank及RDP数据库内同源性较高的细菌16S rDNA序列构建的系统发育树中,分离菌CCF00024与嗜麦芽寡养单胞菌(Stenotrophomonasmaltophilia)聚在一簇,特别是与S.maltophiliaM5-1的同源性最高,其序列相似性达99.6%,结合形态和生理生化特点将其鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)。 A pathogenic bacterium （CCF00024） was isolated from the kidney and liver of the diseased channel catfish with acute epidemic disease. Artificial infection proved that the bacterium was the pathogen of the disease. Its morphological, physiological, biochemical characteristics and 16S rDNA sequence analysis were studied. The isolated strain is an aerobic, non-fermentative bacterium. The bacteria are gram negative, rods, with polar multi-flagella; Oxidase-negative, methyl-red-negative, lysine decarboxylase-positive, DNAase-positive, urease-positive, lipase-positive and protease-positive. The bacteria can＇t utilize most of sugars wfth production of acid, except maltose and mannose. A phylogenetic tree was constructed by comparing the 16S rDNA sequence of the isolated strain （GenBank accession number AY970826） with other relative bacteria species in the RDP and GenBank databases. In the phylogenetic tree CCF00024, Stenotrophomonas maltophilia 13637T, Stenotrophomonas maltophilia MG958T, and Stenotrophomonas maltophilia MS-1 constitute a branch. The similarity value between strain CCF00024 and those 5 strains Stenotrophomonas maltophilia are 99.4% - 99.6%. According to morphological, physiological, biochemical characteristics and phylogenetic analysis, the isolated strain （CCF00024） is identified as Stenotrophomonas maltophilia.

关 键 词： 斑点叉尾鮰 急性流行性传染病 嗜麦芽寡养单胞菌 系统发育

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]