机构地区: 中国热带农业科学院热带作物品种资源研究所
出 处: 《热带农业科学》 2006年第3期11-14,共4页
摘 要: 探讨了影响荔枝花药胚性愈伤组织诱导的因素,通过2,4-D、KT、NAA三因子的正交试验筛选出荔枝花药胚性愈伤组织诱导的最佳植物生长调节剂组合为2.0mg/L2,4-D+2.0mg/LKT。在探讨不同基因型对诱导率影响的基础上,利用流式细胞仪对不同基因型胚性愈伤组织进行了倍性检测。结果表明,不同基因型荔枝花药胚性愈伤组织诱导率存在显著差异,胚性愈伤组织倍性检测结果也不同。 The factors that influenced the rate of anther embryogenic callus induction in litchi were studied. The best combination of plant growth regulators (2.0 mg/L 2,4-D +2.0 mg/L KT) was chosen by using three factors (2,4-D, KT, NAA) orthogonal experiment. According to the effect of different genotypes on the rate of embryogenic callus induction, the ploidy of embryogenic calli originated from different genotypes was detected by using flow cell machine. The ploidy detection indicated that the genotypes performed marked difference in the inducing rates of anther embryogenic calli and produced different results in ploidy detection of embryogenic calli.