作　　者： ; ; ;

机构地区： 解放军第302医院

出　　处： 《色谱》 2006年第4期359-362,共4页

摘　　要： 建立了反相高效液相色谱-蒸发光散射检测法（HPLC—ELSD）同时测定中药阿胶中17种未衍生氨基酸含量的方法。采用Prevail^TM C18色谱柱（250mm×4．6mmi．d．，5μm），以乙腈-0．7％三氟醋酸溶液（含5．0mmol／L七氟丁酸）为流动相进行线性梯度洗脱，流速为0．8mL／min，在漂移管温度115℃、氮气流量2．5L／min条件下，在25min内即可完成对阿胶中17种氨基酸的分离测定。氨基酸质量浓度为0．073～2．327g／L时，其峰面积的对数值与质量浓度的对数值线性关系良好；17种氨基酸的加样回收率为93.5％～104．8％；信噪比为3时，测得氨基酸的最低检测限介于18．2mg／L与54．6mg／L之间。该法快速、简便、准确，可作为阿胶中氨基酸的直接测定方法，亦为其他药物中氨基酸的分析提供了参考。 An analytical method to determine 17 underivatized amino acids in donkey-hide glue was established with reversed-phase high performance liquid chromatography （HPLC） coupled with evaporative light-scattering detection （ELSD）. A Prevail^TM C18 column was used with the mobile phase of acetonitrile-0.7% trifluoroacetic acid containing 5.0 mmol/L heptafluorobutyric acid. Under the condition of solvent gradient elution, the temperature of drift tube was 115 ℃ and the gas flow rate was 2.5 L/min. The 17 amino acids were separated within 25 min. The good linearities between the logarithm of peak area and logarithm of mass concentration of amino acids were obtained in a range of mass concentrations from 0. 073 g/L to 2. 327 g/L. The recoveries of 17 amino acids were 93.5% -104. 8% with the relative standard deviations （RSDs） of 0.58% -2.88%. The lowest detection limits of amino acids were from 18.2 mg/L to 54.6 mg/L with 3 times the signal to noise ratio. This HPLC-ELSD method is rapid, simple and accurate. It can be used for the direct determination of 17 underivatized amino acids in donkey-hide glue. It also serves as a good reference for the determination of amino acids in other fields, such as pharmaceutical analysis.

关 键 词： 反相高效液相色谱 蒸发光散射检测 未衍生化 氨基酸 阿胶

领　　域： [理学] [理学]