作　　者： ; ; ; ;

机构地区： 华南农业大学动物科学学院

出　　处： 《中国预防兽医学报》 2006年第4期401-404,435,共5页

摘　　要： 利用反转录PCR(RT-PCR)与套式PCR技术检测Gal-3基因在鸡体不同组织的分布表达。实验结果表明Gal-3基因在法氏囊、皮肤、舌头、肺脏、骨髓、气管等组织中广泛分布,在肾脏、脾脏、肝脏、胰脏等组织中没有检测到。对从鸡骨髓中扩增出来的β-防御素(Gal-3)cDNA进行克隆测序表明扩增出的cDNA碱基数为297bp,与GenBank中的Gal-3cDNA序列完全相同。核苷酸序列比较表明在同源性上,Gal-3与Gal-1、Gal-2基因核苷酸相似性分别为75%、50%,同火鸡GPV-1基因同源性最高,达91%。体外诱导表达实验表明在气管上皮细胞中LPS与灭活卡介苗可以诱导Gal-3的表达;Gal-3在法氏囊细胞、肺上皮细胞的表达为持续性表达。 The distribution and expression of chicken β-defensin-3（Gallinacin -3, Gal-3） in the tissues of normal chicken were investigated by reverse transcription-polymerase chain reaction （RT-PCR） and nest-PCR. In bursa of Fabricius＇, skin, tongue, lung, borrow and trachea, the Gal-3 mRNA was detected, but not detected in the spleen, kidney, liver and pancreas. The Gal-3 cDNA fragments amplified by RT-PCR from the bone marrow cells of Yuehuang chicken were cloned and sequenced. The result showed the obtained 297 bp DNA fragment is identical to the Gal-3 cDNA sequence registered in GenBank. Gal-3 compared with Gal-land Gal-2, the percent similarities of nucleotide were 75 %, 50 %, respectively. Gal-3 and GPV-1 had the highest percent similarities of nucleotide and putative amino acid sequence among the β-defensins registered in the GenBank, were 91% and 72.7 %, respectively. The expression of Gal-3 in the cultured lung epithelia cells were induced by addition of the LPS and BCG in vitro. Expression of Gal-3 in the Fabricius＇ bursa cells and trachea epithelia cells were constitutive.

关 键 词： 防御素 克隆 诱导表达

领　　域： [农业科学] [农业科学] [农业科学] [生物学]