机构地区: 甘肃农业大学
出 处: 《甘肃农业大学学报》 2006年第3期16-21,共6页
摘 要: 利用随机扩增多态DNA(RAPD)技术分析番鸭的遗传多样性,20个引物共扩增出164条带,分子量约在200 ~2 000 bp之间,番鸭20个个体间遗传距离为0.4572~0.0177,群体内个体间遗传相似度(F)为0.9823~0.5428,遗传多样性指效(Ho)平均为0.1639.结果表明番鸭DNA的同源性高,品种的纯度保持较好. Random amplified polymorphic DNA (RAPD) was used to estimate the genetic diversity of muscovy,twenty 10 bp single primers sequence were used to amplify genomic DNA. 164 bands of 200~2 000 bp were got. The results showed that the genetic distance(D)was 0. 457~0. 017 7, the genetic similarity among individuals(F)was 0. 982 3 t0. 542 8 and average index of genetic diversity(Ho)of 20 muscovies was 0. 163 9. This indicated that muscovies DNA had high homology, and the breed pure was kept well.