作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《营养学报》 2006年第3期216-220,共5页

摘　　要： 目的:探讨黑米花色苷对ox-LDL引起的血管内皮细胞损伤的保护作用。方法:分别在4种黑米花色苷预孵保护作用下用ox-LDL过氧化处理EVC304细胞株,通过加载GIF滤光片的倒置显微镜观察细胞形态;通过MTT实验,荧光分光光度法和2’,7’-二氯荧光素双醋酸盐(DCFH-DA)荧光探针分别检测细胞活力及体内丙二醛和自由基变化;用流式细胞仪分析细胞周期。结果:四种黑米花色苷能明显减轻ox-LDL对内皮细胞形态的损伤,降低ox-LDL对细胞增殖的抑制作用,显著减少细胞内丙二醛增加量;其中,矢车菊素-3-葡萄糖苷还能显著降低ox-LDL所致自由基的生成量,促进细胞由G1期进入S期,促进细胞增殖,减少细胞凋亡。结论:黑米花色苷对ox-LDL引起的血管内皮细胞过氧化损伤有明显保护作用。 Objective： To investigate the protective effects of four kinds of anthocyanidin extracted from black rice fraction on the vascular endothelial cells treated with ox-LDL. Method： The morphological changes of cells were observed with the inverted microscope equipped with GIF filter. The viability of endothelial cells, the production of MDA and free radicals in cells were determined respectively through MTT test, fluorescent spectrophotometry and DCFH-DA as fluorescence probe. We studied the influence of cyanidin-3-glucoside on the cell cycle of endothelial cells treated with ox-LDL by flow cytometry. Result： Anthocyanidins from black rice could decrease the morphological injury of ox-LDL to endothelial cells and reduce the inhibition of ox-LDL to the proliferation of endothelial cells significantly. Cultured with anthocyanidin before ox-LDL, cells produced less MDA than those treated with ox-LDL directly. Cyanidin-3-glucoside decreased the free radicals in cells and the inhibition of cell proliferation due to ox-LDL attack. Conclusion： Anthocyanidin had the ability to protect cells from the oxidative injury of ox-LDL,probably due to its antioxidative activity.

关 键 词： 黑米 花色苷 氧化应激 内皮细胞

领　　域： [生物学]