作　　者： ; ; ; ; ;

机构地区： 四川大学华西药学院

出　　处： 《生物医学工程学杂志》 2006年第3期640-644,共5页

摘　　要： 建立了一套通过热敏法分离纯化及病毒灭活人脐带血血红蛋白的工艺。对该工艺条件的优化,使得纯化血红蛋白产品纯度及回收率等得到提升,并建立了一套较为完善的纯化血红蛋白质量检测指标。与现有的纯化方式相比,热敏法操作简便,仪器设备造价低廉,纯化与病毒灭活同时进行,得到的纯化产品损失少,纯度高,各项理化指标达到国际水平,为规模制备纯化及病毒灭活的血红蛋白以及血液代用品的研发创造了有利条件。 This study was intended to establish a method for preparation of purified and viral-inactivated placenta hemoglobin. The optimum preparative condition resulted in the up-grading of purity,recovery and so on. A quality control was also established for the purification of hemoglobin. Compared to present purification methods, this method is easy to operate, needs low investment and running cost, and has the advantage of simultaneous operation for purification and viral-inactivation. The resulted hemoglobin had high purity and recovery, and the physicochemical property measured up to that in international reports. So this method is suitable for preparing purified and viral-inactivated hemoglobin on an adequate scale, and is useful for further development of blood substitutes.

关 键 词： 热敏法 血红蛋白 纯化 病毒灭活

领　　域： [化学工程]