作　　者： ; ; ; ; ; ;

机构地区： 中南大学湘雅医学院肿瘤研究所

出　　处： 《生命科学研究》 2006年第2期99-102,共4页

摘　　要： 整体原位杂交(whole-mountinsituhybridization,WMH)已经成为基因表达定位和表达分布模式研究的一种重要手段.该技术能在整体水平上精确地研究胚胎发育过程中基因表达的三维信息,而且为大规模筛选区域及组织特异性候选克隆提供了有利的技术手段.采用体外转录地高辛标记的RNA探针,检测已知基因MDM2在鼠胚胎发育不同阶段的表达模式. Whole-mount in situ hybridization has become a very important tool for studying localization and distribution pattern of gene expression. Also, it has greatly facilitated the precise three-dimensional localization of transcripts from genes whose expression is important during development and the ease and speed with which patterns of gene expression can be visualized makes it an attractive technique for screening large numbers of candidate clones for region- or tissue-specific expression. Here we take the MDM2 RNA probe labeled by DIG-11-UTP to detect the expression pattern in different stages of mouse embryos.

关 键 词： 整体原位杂交 探针 小鼠

领　　域： [生物学]