作　　者： ; ; ; ; ; ;

机构地区： 广州器官移植配型中心

出　　处： 《广州医学院学报》 2006年第1期56-57,63,共3页

摘　　要： 目的:研究K IR3DS1与HLA-BBw4正常广东汉族人群中的分布状态。方法:对92例广东汉族干细胞移植供者以序列特异性引物扩增技术(PCR-Sequence Spec ific Prim er)分析K IR3DS1和HLA-B表型;根据BW特异性分类,对HLA-BBw4第80位氨基酸残基为异亮氨酸者指定为HLA-BBw480ILE;依靠计算机软件进行K IR3DS1和HLA-BBw480ILE统计。结果:实验发现,在广东汉族人群中K IR3DS1、HLA-BBw4、HLA-BBw480ILE的表现频率依次为0.228,0.652,0.543;其中12.0%的个体为HLA-BBw4纯合子,14.1%的个体同时表现HLA-BBW 480ILE和K IR3DS1,另外有8.70%单独表现K IR3DS1而不表现HLA-BBw480ILE。结论:在广东汉族人群中,只有14.1%的个体同时表达HLA-BBw480ILE和K IR3DS1,而单独表达2K IR3DS为18.70%。 Objective： To investigate the distribution of KIR3DS1 and HLA-B^Bw4 in Guangdong Han people. Methods： 92 Guangdong Han bone marrow donors were studied. KIR3DS1 and HLA-B type were determined by polymerase chain reaction-sequence-specific-primer （PCR-SSP）. Denoted HLA-B^Bw4 with isoleucine located on 80th amino residual as HLA-B^Bw480^ILE. Coexpression or sole expression of KIR3DS1 and HLA-B^Bw480^ILE was counted using PC software. R^ults： Expression frequencies of KIR3DS1, HLA-B^Bw4 and HLA-B^Bw480^ILE in these Guangdong Han people were 0.228, 0. 652, 0. 542, respectively. Of the cases, 12.0% were HLA-Bs＇4 homozygotes, 14.1% coexpressed HLA-B^Bw480^ILE and KIR3DS1, and 8.7% expressed KIR3DS1 alone. Conclusion： In these Guangdong Hart people, only 14.1% co-expressed HLA-B^Bw480^ILE and KIR3DS1, and 8.7% expressed KIR3DS1 alone.

关 键 词： 杀伤免疫球蛋白样受体 人类白细胞抗原

领　　域： [生物学]