作　　者： ; ; ; ; ; ; ;

机构地区： 南开大学生命科学学院植物生物学和生态学系

出　　处： 《分子细胞生物学报》 2006年第3期208-216,共9页

摘　　要： 植物类受体蛋白激酶(receptor-likeproteinkinase,RLK)在高等植物生长发育和环境刺激的信号传导中起着重要的作用。本文报告了一个新的大豆类受体蛋白激酶基因的全长cDNA克隆及对其基因结构和功能的初步分析。研究表明该基因序列编码的蛋白包含一个跨膜域、一个具有丝氨酸/苏氨酸激酶活性的胞内域和一个缺少N-末端信号肽的胞外域。采用生物信息学方法分析表明,该基因与一些拟南芥菜类受体蛋白激酶基因具有很高的相似性,这些激酶N-末端都缺少信号序列,属于植物胞质类受体激酶(receptor-likecytoplasmickinase,RLCK)亚家族。因此命名该大豆基因为GmRLCK(GenBankAccessionNo.AY687390)。对GmRLCK激酶域中磷酸化可能性较高的位点进行了预测。RT-PCR的结果表明,GmRLCK在大豆子叶、根、花以及豆荚中都有较高的表达,而在胚根、茎和成熟叶片中的表达相对较弱。进化分析表明GmRLCK与一些衰老相关的植物类受体蛋白激酶具有较近的亲缘关系。 Protein kinases play key roles mental signals in higher plants. Here reported in transduction of both environmental and developfull-length cDNA cloning and preliminary structural and functional analysis of a novel soybean receptor-like protein kinase （RLK） gene （GenBank Accession No.AY687390）. The deduced protein product of this gene contains a transmembrane region,an intracellular catalytic domain possessing serine/threonine kinase activity,and an extracellular domain which lacks the N-terminus signal peptide. Bioinformatic analyses revealed highly similarity between this soybean gene and a group of the Arabidopsis RLK genes which all lack of N-terminus signal sequence and belong to RLCK subfamily. Thus,it was designated GmRLCK. Some residues of GmRLCK that showed high probability to be phosphorylated were also predicted. RT-PCR analysis revealed higher expression levels of GmRLCK in soybean cotyledons,roots,flowers and young pods;whereas lower expression levels were detected in radicles,stems and mature leaves at vegetative stage. Phylogeny analysis suggested that GmRLCK was more close to and might have a common ancestor with several senescence-associated RLKs.

关 键 词： 大豆 类受体蛋白激酶 胞质类受体激酶 结构预测 反转录 进化分析

领　　域： [生物学] [农业科学] [农业科学] [农业科学]