作　　者： ; ; ;

机构地区： 暨南大学生命科学技术学院生物工程学系

出　　处： 《广西植物》 2006年第3期300-303,共4页

摘　　要： 利用RAPD技术从大白菜细胞质雄性不育保持系3411-7的DNA中得到一个特异扩增片段MOPB04600。回收该特异扩增片段并将其克隆到pGEM-TEasy载体上进行序列测定。结果表明该片段全长600bp,其碱基组成为A+T=72.33%。通过与GenBank+EMBL+DDBJ+PDB中的455,972个序列进行同源性比较,同源性均小于30%,表明该片段为一新发现的序列。并对该特异片段的来源及其可能的作用进行探讨。 A specific amplified DNA fragment was obtained from cytoplasmic male sterility maintainer line ＂3411-7＂of Chinese cabbage by RAPD technique. This specific fragment was cloned into pGEM-T Easy vector and DNA sequence was determined. The result showed that it was consisted of 600bp and the base component of A＋T was 72.33%. Comparison between MOPB04600 and 455,972 sequences from GenBank＋EMBL＋ DDBJ＋PPB revealed that the homology were low（less than 30%）. This implied that it was a newly found DNA sequence. The origin of MOPB04600 and its possible effects were discussed.

关 键 词： 大白菜 细胞质雄性不育 克隆 测序

领　　域： [生物学]