作　　者： ; ; ; ; ;

机构地区： 东北农业大学动物医学学院

出　　处： 《中国兽医学报》 2006年第3期307-309,共3页

摘　　要： 应用碱性单细胞凝胶电泳技术（single cell gel electrophoresis，SCGE）]检测了0～30μmol／L CdCl2对体外培养的鸡脾脏淋巴细胞DNA的损伤效应。结果表明，镉浓度在0～30μmol／L范围内，随着镉浓度的增加，对鸡脾脏淋巴细胞DNA的损伤作用逐渐增强，呈现显著的剂量效应（r=0．983，P=0．004）。结果提示，镉致鸡脾脏淋巴细胞DNA损伤是镉对禽类免疫毒性的重要机制之一。 Alkaline single cell gel electrophoresis test was used to study DNA damage of chicken splenic lymphocytes cultured in vitro which was induced by different concentration of cadmium chloride from 0 to 30 μmol/L. The result showed that the chicken splenic lymphocytes DNA damage induced by cadmium gradually enhanced with the increasing concentration of cadmium within a range from 0 to 30 μmol/L, which showed a dose-dependent model （r = 0. 983,P = 0. 004）. It suggests that lymphocytes DNA damage induced by cadmium may be one of important mechanism on the avian immunotoxic mechanisms of cadmium.

关 键 词： 镉 鸡脾脏淋巴细胞 单细胞凝胶电泳 损伤

领　　域： [农业科学] [农业科学] [农业科学] [生物学]