作　　者： ; ; ; ; ; ; ;

机构地区： 清华大学

出　　处： 《分析试验室》 2006年第5期23-27,共5页

摘　　要： 建立了大鼠急性心肌梗死模型, 利用二维电泳（2DE）分离正常与梗死心肌组织的蛋白, 得到不同条件下的蛋白质组图谱. 在pH 5～8范围下采用银染方法通常可以检测出超过800个蛋白点. 对比正常心肌组织, 梗死组织31个蛋白表达上调, 18个蛋白表达下调. 我们选择其中具有显著性变化的18个蛋白进行质谱分析, 并通过数据库检索, 成功鉴定了其中的8个蛋白. 其中, Hsp27, HspB6, 超氧化物歧化酶[Cu-Zn], 阿朴脂蛋白A-I, 在急性心肌梗死发病过程中起重要的作用, 可以作为临床诊断的重要标志物, 为急性心肌梗死的治疗提供药物作用靶点. The animal model of acute myocardial infarction was established by surgical ligation of the left coronary artery in rats. Two-dimensional gel electrophoresis （2-DE） profiles of normal and infarcted cardiac muscle tissues were compared; over 800 protein spots were separated in the silver-stained gels with the pH range of 5 - 8. The results showed that the expressions of 31 spots were increased and 18 spots decreased in the diseased tissues compared with normal tissues. 18 altered proteins were subjected to mass spectrometry analysis, and 8 proteins were subsequently identified with peptide mass fingerprinting based on matrix-associated laser desorption ionization time-of-flight mass spectrometer （MALDI-TOF-MS） and database searching. These proteins, Hsp27, Hsp20, superoxide dismutase [Cu- Zn], apolipoprotein A-Ⅰ, may be involved in the acute myocardial infarction and play an important role as potential markers for the future diagnosis and treatment of cardiovascular disease.

关 键 词： 急性心肌梗死 蛋白质组 二维电泳 质谱

领　　域： [理学] [理学]