作　　者： ; ; ; ; ;

机构地区： 南方医科大学

出　　处： 《生物化学与生物物理进展》 2006年第5期485-491,共7页

摘　　要： 采用对同一亲本来源、不同转移潜能细胞株SW480和SW620的蛋白质表达谱进行双向凝胶电泳和质谱技术分析,并在蛋白质和mRNA水平进行验证,成功鉴定了10个大肠癌转移相关蛋白,其中SW620细胞株表达上调的蛋白质有磷酸甘油酸变位酶1,磷脂酰乙醇胺结合蛋白和高迁移率族蛋白B-1,而热休克蛋白27,膜联蛋白Ⅰ,甲硫腺苷磷酸化酶,切丝蛋白1和表皮型脂肪酸结合蛋白在SW620中表达下调.大多数差异蛋白质功能涉及肿瘤细胞生长、运动、粘附、凋亡等过程,研究结果为阐明大肠癌转移机制及寻找预测大肠癌转移的潜在标志物提供了理论依据. Two-dimensional （2D） gel electrophoresis followed by matrix-assisted laser desorption/time of flight mass spectrometry was utilized to compare the protein expression profiles between two colorectal cancer （CRC） cell lines with high and low metastatic potentials, SW620 and SW480. 10 metastasis-associated proteins were identified and further validated by Western blotting and/or semi-quantitative RT-PCR analysis. Of the identified proteins, the expressions of phosphoglycerate mutase 1, phosphatidylethanolamine binding protein and high-mobility group box 1 were elevated in SW620 cells. However, heat shock protein 27, annexin Ⅰ , methylthioadenosine phosphorylase, cofilin-1 and epidermal fatty acid binding protein were down-regulated in SW620 cells. Most of the candidate proteins have been evident to be somehow associated with various aspects of tumor metastasis such as cell growth, motility, invasion, adhesion, apoptosis and tumor immunity. These results provide the basis for searching for potential markers for CRC prognosis and give some clues to elucidate the mechanism of CRC metastasis.

关 键 词： 大肠癌 转移 蛋白质组 双向凝胶电泳

领　　域： [生物学] [生物学]