作　　者： ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《陕西科技大学学报（自然科学版）》 2006年第2期13-20,共8页

摘　　要： 研究了谷氨酰胺转移酶(TGase)对大豆分离蛋白(SPI1和SPI2)、酪蛋白酸钠(NaCas1和NaCas2)及明胶蛋白质(G1和G2)成膜特性的影响。研究表明,在成膜溶液中加入TGase(8U/g.蛋白)可以使SPI、NaCas和明胶等蛋白质膜的抗拉强度与表面疏水性得到不同程度的改善,其中SPI2膜、G1膜、SPI1膜、NaCas2膜和NaCas1膜的抗拉强度分别增加了33.2%、25.6%、17.7%、17.6%和13.1%(p≤0.05),NaCas1膜、SPI1膜、NaCas2膜和G1膜的表面疏水性分别增加了216.1%、116.9%、33.1%和2.4%(p≤0.05),同时也明显降低了膜的水分含量、总可溶性物量及透光率。对于断裂伸长率,TGase的处理使G1膜、NaCas2膜、G2膜、NaCas1膜和SPI2膜分别增加了16.3%、16.8%、43.0%、72.6%和440.5%,而使SPI1膜降低了7.5%。SDSPAGE电泳分析表明,TGase使这6种蛋白质均产生了共价交联。 Effects of transglutaminase （TGase） treatment on properties of cast films from soy protein isolates （SPI-1, SPI-2）, sodium caseinate （NaCas-1, NaCas-2） and gelatin （G-1, G- 2） were investigated. Tensile strength （TS）, elongation at break （EB）, moisture content （MC）, total soluble matter （TSM）, surface hydrophobicity （So） as well as transparency of TGase-treated films and control films were evaluated after conditioning film specimens at 25 ℃ and 50% relative humidity （RH） for 48 h. TGase treatment significantly increased （p≤ 0.05） the TS and So of the most of protein films by 13.1%-33.2% and 2.4%-216.1%, respectively, and simultaneously significantly decreased the TSM of all films by 21. 2% -69.9%. TGase treatment improved the EB of films cast from SPI-2, NaCas and gelatin, while reduced the EB of SPI-1 films. Transparency of TGase-treated films from SPI-1, Na- Cas-2 and G-1 was lower than that of control. SDS-PAGE analyses confirmed the occurrence of cross-linking induced by TGase, which may account for the higher TS, So and lower TSM （as compared to those of the control）.

关 键 词： 谷氨酰胺转移酶 蛋白质膜 膜性能 交联

领　　域： [生物学]